CYTO U Courses
Collapse Flow Cytometry Biosafety Course

Authors: Kevin Holmes, Steve Perfetto, Hank Pletcher, Ingrid Schmid

The Flow Cytometry Biosafety course will provide a summary of biosafety principles as they apply to flow cytometry and cell sorting and provide an overview of applicable standards and risk assessment. The topics covered will be:

  • Biosafety Principles and Procedures
  • Understanding Risk Assessment
  • Instrument-specific Standard Operating Procedures (SOPs)
  • Biosafety in Cell Sorting
  • Containment Testing

At the completion of this course, students will have a clearer understanding of the principles and practices of biosafety as it pertains to flow cytometry, in particular cell sorting. Additionally, you will have a list of resources to aid in risk assessment and the development of Standard Operating Procedures in your own lab. 

To view the course, Click "Register Now."

If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.  
 
For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can re-set it on the ISAC Web site by clicking on the "Forgot Password?" link on the log-in page.
Formats Available: Streaming

Approved Credit:
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Flow Cytometry Biosafety Course
    Collapse Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle

    Authors: Paul K. Wallace, Kathy Muirhead and Kylie Price

    Course Objectives:

    • To understand what cellular processes need to occur in order for a cell to enter the cell cycle and complete division.
    • To know and understand the difference between cell cycle progression and cell division
    • To understand how flow cytometry is used to measure cell cycle parameters and division

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    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.  
     
    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can re-set it on the ISAC Web site by clicking on the "Forgot Password?" link on the log-in page.
     
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    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit.  ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.
     
    Formats Available: Streaming
    Original Seminar Date: September 02, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle
    Collapse Calibration, Lesson 1:  Overview and Checklist for Benchmarking
    Image Cytometer Characterization and Calibration - Lesson 1 of 3

     

    Welcome to the Image Cytometer Characterization and Calibration course.  This course contains three lessons.  The first lesson is "Overview and Checklist for Benchmarking". 

    This course has been developed for core facility managers, clinical personnel involved in quality assurance and scientists interested in reproducibility of imaging measurements.  Students should have a basic understanding of optical microscopy and hands-on experience with a fluorescence microscope.

    Formats Available: Online Course
    Original Seminar Date: April 10, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Calibration, Lesson 1:  Overview and Checklist for Benchmarking
    Collapse Proliferation Monitoring, Lesson 2A: Principles and Methods

    “Proliferation Monitoring by Flow Cytometry”

    The first module in Lesson 2 of the course series

    Lesson 2:   Monitoring Cell Cycle Progression

    Module A:  Principles and Methods

    This is the first of four modules of Lesson 2 of the Proliferation course.  Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

    1. How cells within a population of interest are distributed among the different cell cycle phases; and
    2. The kinetics with which cells of interest are progressing through the cell cycle

    Lesson 1: Basics of the Cell Cycle is currently available here.

    Authors

    Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

    Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

    Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

    Target Audience

    Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle.

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

     

    Formats Available: Proliferation Monitoring - Lesson 2A - Principles and Methods
    Original Seminar Date: August 30, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2A: Principles and Methods