Cell Sorting for Function and Viability
Monica DeLay, Cincinnati Children's Hospital, USA
Peter Lopez, NYU School of Medicine, USA
Matthias Schiemann, Technical University of Munich, Germany
The objective of a successful sort is to obtain viable cells functionally representative of the unmanipulated target population. Towards this goal, there are a number of factors to consider depending on cell type and downstream application. This tutorial will review basic guidelines (i.e. buffers, nozzle size, pressure) that are broadly applicable for obtaining useful sorted product. Specific examples where conditions were optimized for certain cell types will be highlighted. Methods to ascertain cell perturbation as a result of sorting will also be discussed. In addition, attendees are encouraged to bring their relevant experiences to share.