Multiplexed Fluorescence Microscopy Reveals Heterogeneity Among Stromal Cells in Mouse Bone Marrow Sections
Monday, April 15th, 2019 at 12:00 pm EST
Anja E. Hauser, PhD
Charité – Universitätsmedizin and Deutsches Rheumaforschungszentrum Berlin, Germany
Kewal Asosingh, MS, PhD, SCYM(ASCP)CM
Associate Professor of Molecular Medicine
Scientific Director Flow Cytometry Core
Lerner Research Institute
Cleveland Clinic Foundation
About the Faculty
During her studies of veterinary medicine, Anja Hauser developed an interest in immunopathology and microscopy. As a graduate student, she identified factors which attract plasma blasts into the bone marrow and keep them alive in specialized niches within this tissue. During her postdoctoral work, she worked on the migration of germinal center B cells using intravital 2-photon microscopy. Since 2011, she is professor for immune dynamics and intravital microscopy at the Charité – Universitätsmedizin and Deutsches Rheumaforschungszentrum Berlin. In an interdisciplinary approach, her lab develops novel microscopy technologies in order to obtain a deeper insight how the immune system function.
I will introduce the principles of multi epitope ligand cartography, a method for multiplexed immunofluorescence microscopy, and explain its application for analyzing complex tissues and rare cell subsets. I will also give an overview on methods suitable to quantitatively analyze those complex multi-parametric image data.
-Describe MELC as a method for multiplexed immunofluorescence histology
-Learn what to consider when preparing tissues for MELC analysis, choosing marker panels and planning the sequential staining in the tissue.
-Learn about options for image analysis in order to extract quantitative information from multiplexed immunofluorescence histology.
Who Should Attend
Everyone who is interested in quantitative multiplexed image analysis and its applications in immunology