In a scientific tutorial in 2011, one of us (W.T.) covered the fluorescent proteins (FPs) available at the time. As a follow-up, we will focus on recent developments in the field, including new FPs, new lasers, and the advances made in detecting FPs using spectral flow cytometry. We will discuss considerations for practical use of FPs and make recommendations for choice of FPs in flow cytometry. We will analyze FP data using fluorescence compensation for conventional flow cytometry and unmixing algorithms for spectral flow cytometry. Attendees are encouraged to bring along their own data. Upon completion of this tutorial, the attendee will have an overview of the properties of commonly used FPs, the pertinent information to create optimal combinations of FPs, the knowledge to devise feasible detection strategies, and the skill to analyze FP data.
Course Details or Outline:
International Society for Advancement of Cytometry
American Society for Clinical Pathology
This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1.5 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.
ISAC Member Price: $0.00