Research projects frequently encompass technologies from more than one Shared Resource Lab (SRL). Novel applications, such as single-cell RNASeq downstream of cell sorting, may require the involvement of several SRLs from sample collection to data analysis. Often little to no communication between the client and the core staff occurs with regards to the experimental goals or the upstream and downstream processes related to a service request. This tutorial will discuss applications for which multiple core technologies are needed to complete a research project and strategies for effective communication, cooperation and coordination between staff of different cores. Upon conclusion of the tutorial, attendees will have specific tools to aid in the integration of new technologies from other cores into investigator-driven projects that utilize their SRL.
Research projects and applications using cytometry techniques that require cross-core collaboration such as cell sorting to single cell RNASeq using the Fluidigm C1 Critical parameters for experimental success Strategies for interaction between cores to support the investigator.