Monica DeLay, MS
After receiving her master’s degree in biology, she performed research for over a decade in the field of autoimmune diseases focusing on the structure and function of human MHC and its role in modulating disease. Throughout that time she used flow cytometry for her research and in 2008 began managing the Research Flow Cytometry Core at Cincinnati Children’s Hospital in Cincinnati, Ohio. Over the last seven years she has helped facilitate the growth of the core in instrumentation, infrastructure, staff and clientele. She is co-founder and president of the Ohio River Valley Cytometry Association, a local organization connecting advancements in cytometry technology to local scientists. She is co-chair of the ABRF Flow Cytometry Research Group whose mission is to understand alterations in cell function and develop best practices associated with cell sorting. She is a member of the organizing committee for the Core Managers workshop for the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) and serves as a council member for the International Society for Advancement of Cytometry (ISAC).
Sherry Thornton, Ph.D.
Dr. Thornton received her Ph.D. in Developmental Biology from the University of Cincinnati College of Medicine. Her postdoctoral fellowship involved the use of flow cytometry in antigen-specific responses in the laboratory of Dr. Raphael Hirsch. She then joined the faculty in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center (CCHMC) and became flow core director for the Rheumatology Center Grant (NIH P30 AR47363). She has been extensively involved with the correlation of gene expression profiles with cell types (determined by flow cytometry analysis) of PBMC from large cohorts of Juvenile Idiopathic Arthritis patients (>500) and control specimens. Her research interests also include examination of cell types involved in the pathogenesis of arthritis. In June of 2008, Dr. Thornton led the consolidation of flow cytometry at CCHMC, and she became Director of the Research Flow Cytometry Core that houses all flow cytometry instrumentation involved in basic research at CCHMC. Dr. Thornton is active locally, nationally and internationally in the flow cytometry community. She is an active member of the International Society for Advancement of Cytometry, a founding member of the Ohio River Valley Cytometry Association, steering committee member of the Great Lakes International Imaging and Flow Cytometry Association and a member of the Career Development Committee of the Association of Biomolecular Resource Facilities.
Novel applications, such as single-cell RNASeq, induced pluripotent stem cell (iPSC) generation and CRISPR-mediated genome editing, have revolutionized the way that cells can be analyzed and manipulated to investigate developmental pathways and disease processes. These technologies can also benefit from using flow cytometry as an upstream or downstream process and, when combined, may require the involvement of several Shared Resource Labs (SRLs) from sample collection to data analysis. This webinar will highlight research projects that have utilized the above applications in combination with flow cytometry and/or cell sorting, discuss critical parameters that should be considered for successful experimental outcomes and provide strategies for coordination among core facility staff and investigators. This discussion is aimed to provide ideas on the integration of new technologies from other cores into investigator-driven projects that utilize their SRL.
This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.