Considerations for Integrating Flow Cytometry with Single Cell Transcriptomics

In recent years, technical advances have made it possible to obtain transcriptomic data (i.e., mRNA expression) from single cells.  This presentation will be focused on using this technology for studies of T-cells and will discuss:  a) stimulation of cells; b) purification and sorting of single cells; c) considerations for selecting mRNA targets and qualifying assay primers/probes; d) procedures and workflow for performing the assay; and e) data analysis considerations.  We will also discuss the power and limitations of transcription-based approaches to cytometry.

 

Course Details/Objectives:

Overview of why transcriptomics might be valuable in cytometry.  Methods for stimulation of cells, purification/sorting of cells, QC of assay and performing pre- and on-chip amplifications.  Data Analysis.  Summary/Conclusions.

Seminar Information
Seminar Date:
June 11, 2016
Single Cell Genomics
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.50
Products
Considerations for Integrating Flow Cytometry with Single Cell Transcriptomics
ISAC Member Price: $0.00
Non-Member Price:$37.50