The word "Cytometry" is a fusion of two Greek words, "kytos" meaning "basket/cell" and "metria" meaning to "measure". Cytometry itself can be conducted in many ways but whatever the chosen approach, it almost always involves measuring the properties of single cells/particles from within large populations with the sole aim of decoding the heterogeneity inherent to all biological systems. While Non-Image-based Flow Cytometry (NIFC) is a powerful, multi-parameter, high-throughput cytometric technology with widespread applications, it cannot provide the spatial/morphometric information often essential to addressing key biological questions. As such a number of imaged-based cytometric approaches exist that can be grouped into a relatively broad field known as "Imaging Cytometry" (IC). This tutorial is primarily aimed at cytometrists who have a background in NIFC and want to understand the basic principles of IC and how it can be pivotal in decoding biological heterogeneity at the cellular level. The course will provide a generic definition and the underlying principles of IC as well as outlining the key stages in a typical IC experimental pipeline. Application examples will be included where appropriate in order to provide context to the participants. A recurring theme throughout the tutorial will be to compare and contrast IC with NIFC in order to provide a foundation for introducing IC as a potentially new concept to the attendees by building on their existing knowledge/experience.