Course overview: Microscopy experiments have proven to be a powerful means of generating information-rich data for biological applications. From small-scale microscopy experiments to time-lapse movies and high-throughput screens, automatic image analysis is more objective and quantitative and less tedious than visual inspection. This course will introduce users to the free open-source image analysis program CellProfiler and its companion data exploration program CellProfiler Analyst.  We will show how CellProfiler can be used to analyse a variety of types of imaging experiments.  We will also briefly discuss the basic principles of supervised machine learning with CellProfiler Analyst in order to score complex and subtle phenotypes.
Outline:
•    Basics of image analysis
•    Methods of distinguishing objects of interest from the image background
•    Methods of separating clusters of touching objects
•    Obtaining measurements from the cell 
•    Exporting measurements and images

Seminar Information
Date Presented:
June 10, 2017 12:00 AM Eastern
Length:
No Longer Available
Introduction to CellProfiler, Free Open-Source Software for Image Analysis

Course overview: Microscopy experiments have proven to be a powerful means of generating information-rich data for biological applications. From small-scale microscopy experiments to time-lapse movies and high-throughput screens, automatic image analysis is more objective and quantitative and less tedious than visual inspection. This course will introduce users to the free open-source image analysis program CellProfiler and its companion data exploration program CellProfiler Analyst.  We will show how CellProfiler can be used to analyse a variety of types of imaging experiments.  We will also briefly discuss the basic principles of supervised machine learning with CellProfiler Analyst in order to score complex and subtle phenotypes.
Outline:
•    Basics of image analysis
•    Methods of distinguishing objects of interest from the image background
•    Methods of separating clusters of touching objects
•    Obtaining measurements from the cell 
•    Exporting measurements and images

Speaker Information
Beth Cimini  [ view bio ]
Kyle Karhohs  [ view bio ]
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CYTO 2017 Scientific Tutorial
ISAC Member Price: $0.00
Non-Member Price:$37.50
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.50
Products
CYTO 2017 Scientific Tutorial
ISAC Member Price: $0.00
Non-Member Price:$37.50