Course Overview:

I.   Overview and Objectives

      a.  Why calibrate? Why characterize?

      b.  Types of calibrators and standards

II.   Calibration of the Fluorescence Intensity Scale

      a.  Photoelectrons

      b.  Fluorophores: MESFs and ERFs

      c.  Molecules: Antibodies and ligands

III.  Characterization of Instrument Performance

      a.  Definitions of detection limit

      b.  Measuring detection efficiency (Q) and background (B)

      c.Limit of resolution/detection

IV.  Practical Applications

      a.  Instrument evaluation and comparison

      b.      Routine QC and SRL best practices

      c.      Implications for polychromatic panel design

     d.      Measurement of small, dim particles

Seminar Information
Seminar Date:
June 10, 2017
Flow Cytometer Calibration and Characterization

I.    Overview and Objectives

      a.  Why calibrate? Why characterize?

      b.  Types of calibrators and standards

II.   Calibration of the Fluorescence Intensity Scale

      a.  Photoelectrons

      b.  Fluorophores: MESFs and ERFs

      c.  Molecules: Antibodies and ligands

III.  Characterization of Instrument Performance

      a.  Definitions of detection limit

      b.  Measuring detection efficiency (Q) and background (B)

      c.  Limit of resolution/detection

IV.  Practical Applications

      a.  Instrument evaluation and comparison

      b.  Routine QC and SRL best practices

      c.  Implications for polychromatic panel design

     d.  Measurement of small, dim particles

Speaker Information
John Nolan  [ view bio ]
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.50
Products
CYTO 2017 Scientific Tutorial
ISAC Member Price: $0.00
Non-Member Price:$37.50