Proliferation Monitoring, Lesson 2A: Principles and Methods

Proliferation Monitoring by Flow Cytometry

The first module in Lesson 2 of the course series

Lesson 2: Monitoring Cell Cycle Progression

Module A: Principles and Methods

This is the first of four modules of Lesson 2 of the Proliferation course.  Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

  1. How cells within a population of interest are distributed among the different cell cycle phases; and
  2. The kinetics with which cells of interest are progressing through the cell cycle

Lesson 1: Basics of the Cell Cycle is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.


Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

Target Audience

Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle.

CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.


Seminar Information
Seminar Date:
August 30, 2017
Proliferation Monitoring - Lesson 2A - Principles and Methods
ISAC Member Price: $0.00
Non-Member Price:$0.00
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
ISAC Member Price: $0.00
Non-Member Price:$25.00