Proliferation Monitoring, Lesson 2A: Principles and Methods

Proliferation Monitoring by Flow Cytometry

 

The first module in Lesson 2 of the course series

 

Lesson 2: Monitoring Cell Cycle Progression

Module A: Principles and Methods

 

This is the first of four modules of Lesson 2 of the Proliferation course.  Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

 

  1. How cells within a population of interest are distributed among the different cell cycle phases; and
  2. The kinetics with which cells of interest are progressing through the cell cycle

 

Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wish to review) how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle is encouraged to attend.

 

Lesson 1: Basics of the Cell Cycle is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

 

Authors:

 

Kylie M. Price

Hugh Green Cytometry Core Facility

Malaghan Institute of Medical Research

Wellington, New Zealand

 

Katharine A. (Kathy) Muirhead

SciGro, Inc., Midwest Office

Middleton, Wisconsin, USA

 

Paul K. Wallace

Department of Flow and Image Cytometry,

Roswell Park Cancer Institute

Buffalo, New York, USA

 

 

To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

 

If you are not logged in to the CYTO U website, you will be directed to the ISAC website to enter your login information.

 

For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

 

This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

 

 

 

 

Seminar Information
Seminar Date:
August 30, 2017
Proliferation Monitoring - Lesson 2A - Principles and Methods
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American Society for Clinical Pathology
CMLE: 1.00
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